Those of you who would like to study mycelium and can't find agar, can't afford it, or whatever your reason may be, can use this little technique.
Ingredients:
Preparation of media(for each jar):
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For a thinner layer:
To inoculate with a spore syringe (multispore) simply flame needle,
wipe with alcohol, and plunge syringe in through the polyfill, tyvek and
inject 1 (one) drop of spore solution in the center of media.
Remove syringe, replace foil and incubate at 80°F - 85°F.
This colonizes 100% in about 2 weeks or less (less using the thin layer) .
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Once you have the growth you are looking for you can transfer a wedge to the grain of your choice.
This is has worked well for me, and I hope you can find it useful.